3D Cell Culture by Zuzana Koledova

Author:Zuzana Koledova
Language: eng
Format: epub
Publisher: Springer New York, New York, NY

2.4 Immunofluorescence Staining and Confocal Microscopy

1.4% paraformaldehyde (PFA) in PBS.

2.Wash solution (PBS-T): 0.5% Triton X-100 in PBS.

3.Blocking solution: 5% normal goat serum (NGS) (Sigma, G9023) in PBS-T.

4.Mouse monoclonal anti-glial fibrillary acidic protein (GFAP) antibody (Sigma, G3893), 1:200 dilution in PBS-T.

5.Goat anti-mouse IgG,F(ab′) 2-TRITC antibody (Santa Cruz, sc-3796), 1:100 dilution in PBS-T.

6.Isolectin GS-IB4 from Griffonia simplicifolia, Alexa Fluor 647 conjugate (ILB4-Alexa Fluor 647) (Life Technologies, I32450), 1:500 dilution in PBS-T.

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